We used scalation, color pattern, and scanning electron microscopy of scale microdermatoglyphics to evaluate the hypothesis that an unusual rattlesnake specimen, from Wise Co., Texas, represents a natural hybrid between Crotalus atrox and C. horridus. The specimen demonstrates particular features of scalation and color pattern that are characteristic of either, but not both, parental species. With respect to microstructure of scales, the specimen exhibits a phenotype intermediate between the putative parental species. Based on this evidence, we tentatively consider the specimen to represent the first reported natural hybrid between C. atrox and C. horridus.